Generator

Part:BBa_K1179040:Design

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)


TRE_Cas9-VP16


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 3695
    Illegal XbaI site found at 30
    Illegal PstI site found at 2084
    Illegal PstI site found at 2318
    Illegal PstI site found at 3530
    Illegal PstI site found at 3834
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 3695
    Illegal PstI site found at 2084
    Illegal PstI site found at 2318
    Illegal PstI site found at 3530
    Illegal PstI site found at 3834
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 3695
    Illegal BglII site found at 1060
    Illegal BamHI site found at 1878
    Illegal XhoI site found at 3416
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 3695
    Illegal XbaI site found at 30
    Illegal PstI site found at 2084
    Illegal PstI site found at 2318
    Illegal PstI site found at 3530
    Illegal PstI site found at 3834
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 3695
    Illegal XbaI site found at 30
    Illegal PstI site found at 2084
    Illegal PstI site found at 2318
    Illegal PstI site found at 3530
    Illegal PstI site found at 3834
    Illegal NgoMIV site found at 2612
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For people interested in using this part, the Cas9-VP16 has only been used as a transactivator on a minimal CMV promoter.

Source

Constructed by performing a Gateway reaction of the TRE-tight promoter and the Cas9-VP16 entry vector. The Cas9 protein originates from the type II prokaryotic CRISPR system in the bacterium Streptococcus pyogenes.

References